HISTOPATHOLOGICAL PATTERNS OF LESIONS CAUSED BY TWO DICHORHAVIRUS
DOI:
https://doi.org/10.37856/bja.v95i3.4263Resumo
 Different from most plant viruses, Brevipalpus mite-transmitted viruses (BTV) cause localized infection, not being able to systemically invade the infected plant. To broaden the specter of the histopathological changes in leaf tissues induced by BTV infection, we examined the tissue organization of leaf lesions caused by the infection of two additional representatives of dichorhaviruses, Clerodendrum chlorotic spot virus (ClCSV) and Coffee ringspot virus (CoRSV). In general, tissue alterations within the lesions followed the pattern noticed in CiLV-C and CiLV-N infection.  In all analyzed lesions, necrotic cells form the central region that is surrounded by a chlorotic halo where hypertrophied cells commonly occur. Presence of reactive oxygen components were also a characteristic of the chlorotic halo. Though more cases of localized lesions on leaves infected by more BTV, remains to be analyzed, it seems that these lesions respond similarly upon infection by BTV, either Cile- or Dichorhavirus.ÂReferências
REFERENCES
APEZZATTO-DA-GLÓRIA, B., MARQUES, J.P.R., FREITAS-ASTÚA, J. & KITAJIMA,E.W. Análise morfológica da evolução das lesões lepróticas foliares de Citrus sinensis (L.) Osbeck ‘Pera’. Revista Brasileira de Biociências Porto Alegre, vol. 5, supl.:879-881.2007.
ARENA G.D., RAMOS-GONZÃLEZ, P. L., FALK B.W., CASTEEL C. L., FREITAS-ASTÚA J., MACHADO M.A. 2020.Plant immune system activation upon citrus leprosis virus c infection is mimicked by the ectopic expression of the P61 viral protein . Frontiers in Plant Science, Parkville, DOI: 10.3389/fpls.2020.01188
BASSANEZI, R.B. 2018. Mudanças na citricultura e o controle do ácaro da leprose. Citricultura Atual, Cordeirópolis, pp. 21-24 .2018.
CALEGARIO, R. F., GARITA, L., SALAROLI, R.B., KITAJIMA, E.W. Immunofluorescence studies on the distribution of Citrus leprosis virus C (CiLV-C) in different hosts. Res. XIII Congresso Soc.Bras.Microscopia e Microanálise, 2011, Búzios, RJ. Soc. Bras. Microscopia e Microanálise. v. 1.2011.
CHABI-JESUS, C., RAMOS-GONZÃLEZ, PL, TASSI, AD, GUERRA-PERAZA, O, KITAJIMA, E, HARAKAVA, R, BESERRA JR, JEA, SALAROLI, RB, FREITAS-ASTUA, J. 2018. Identification and characterization of citrus chlorotic spot virus, a new dichorhavirus associated with citrus leprosis-like symptoms. Plant Disease, St. Paul, vol. 102, pp.1588-1598.
CHAGAS, C.M. 1978. Mancha anular do cafeeiro: transmissibilidade, identificação do vetor e aspectos anatomo-patológicas da espécie Coffea arabica L. afetada pela moléstia. PhD Thesis, São Paulo, Universidade de São Paulo, 132 pp.
CHILDERS, C.C. & DERRICK, K. 2003a. Brevipalpus mites as vectors of unassigned rhabdoviruses in various crops. Experimental Applied Acarology, Amsterdam, vol. 30, pp. 1-231.
CHILDERS, C.C., RODRIGUES, J.C.V., DERRICK, K.S., ACHOR, D.S., FRENCH, J.V., WELBOURN, W.C. , OCHOA, R. & KITAJIMA, E.W. 2003b. Citrus leprosis and its status in Florida and Texas: past and present. Experimental Applied Acarology, Amsterdam, Vol. 30: pp. 181-202.
COOK, G., KIRKMAN, W., CLASE, R., STEYN, C., BASSON, E., FOURIE, P.H., MOORE, S.D., GROUT, T.G., CARSTENS, E., HATTINGH, V. 2019. Orchid fleck associated with the first case of citrus leprosis-N in South Africa. European .Journal Plant Pathology, London, vol. 155, pp. 1373-1379.
DEDECCA, D.M. 1957. Anatomia e Desenvolvimento ontogenético de Coffea arabica L. var. typica Cramer. Bragantia, Campinas, vol. 16: 315-366.
DIETZGEN, R. G.; KUHN, J. H.; CLAWSON, A. N.; FREITAS-ASTÚA, J.; GOODIN, M. M.; KITAJIMA, E. W.; KONDO, H.; WETZEL, T.; WHITFIELD, A. E. 2014. Dichorhavirus: a proposed new genus for Brevipalpus mite-transmitted, nuclear, bacilliform, bipartite, negative-strand RNA plant viruses. Archives in Virology, Wien, vol. 159, pp. 607–19.
DIETZGEN, R.G. FREITAS-ASTÚA, J., CHABI-JESUS, C., RAMOS-GONZÃLEZ, P.L., GOODIN, M.M., KONDO, H., TASSI, A.D., KITAJIMA, E.W. 2018. Dichorhaviruses in their host plants and mite vectors. Advances in Virus Research, New York, vol. 102, doi.org/10.1016/bs.aivir.2018.06.001.
DÃAZ-VIVANCOS, P.; CLEMENTE-MORENO, M.J.; RUBIO, M.; OLMOS, E.; GARCÃA, J.A.; MART ÌINEZ-GÓMEZ, P.; HERNÃNDEZ, P.A. 2008.Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus Journal of Experimental Botany, Lancaster vol. 59, n.. 8, pp. 2147–2160.
FAWCETT, H.S. . 1911. Scaly bark or nail head rust of citrus. Florida Agricultural Exerimental Station Bulletin, Gainesville, vol. 106. 41p
FREZZI M.S. 1940. La lepra explosive del naranjo. Investigaciones realizadas por el laboratorio de patologia de Bella Vista (Corrientes). Boletin Fruticultura e Horticultura, Buenos Aires, n.5, pp. 1-16.
FREITAS-ASTÚA, J., RAMOS-GONZÃLEZ, P.L., ARENA, G.D., TASSI, A.D., KITAJIMA, E.W. Brevipalus-transmitted viruses: parallelism beyond a common vector or convergent evolution of distantly related pathogens? Current Oppinion in Virology, Amsterdan, 2018. DOI 10.1016/j.coviro.2018.07.010
GOMES, R.T.; KITAJIMA, E.W.; TANAKA, F.A.O.; MARQUES, J.P.R.; APPEZZATO-DA-GLÓRIA, B. Anatomia de lesões foliares causadas pelo vÃrus da Mancha Clorótica do Clerodendrum, transmitido pelo ácaro Brevipalpus phoenicis em diferentes espécies. Summa Phytopathologica, Jaguariúna, v.36, n.4, p.291-297, 2010.
HERMAN, P.P.J. 1998. The leaf anatomy of two Clerodendron species (Verbenaceae). South African Journal of Botany, Scottsville, vol. 642, pp. 46–249.
HERNÃNDEZ, J.A.; GULLNER, G.; CLEMENTE-MORENO, M.L.; KÃœNSTLER, A.; SILLA JUHÃSZ S.; DÃAZ-VIVANCOS, P.; KIRÃLY, K. 2016. Oxidative stress and antioxidative responses in plant–virus interactions. Physiological and Molecular Plant Pathology, London, vol. 94, pp. 134-148.
KITAJIMA, E.W., REZENDE, J.A.M. & RODRIGUES, J.C.V. 2003a. Passion fruit green spot virus vectored by Brevipalpus phoenicis (Acari: Tenuipalpidae). Experimental Applied Acarology, Amsterdan, vol. 30, pp. 225-231.
KITAJIMA, E.W., CHAGAS, C.M. & RODRIGUES, J.C.V. 2003b. Brevipalpus-transmitted plant virus and virus-like diseases: cytopathology and some recent cases. Experimental and Applied Acarology, Amsterdan, vol. 30, pp.135-160,
KITAJIMA, E. W., RODRIGUES, J. C. V., FREITAS-ASTUA, J. 2010b.An annotated list of ornamentals naturally found infected by Brevipalpus mite-transmitted viruses. Scientia AgrÃcola, Piracicaba, vol. 67, n.3, p.348-371.
KITAJIMA, E.W., CHAGAS, C.M., HARAKAVA, R., CALEGARIO, R.F., FREITAS-ASTÚA, J., RODRIGUES, J.C.V. & CHILDERS, C.C. 2011a. Citrus leprosis in Florida, USA, appears to have been caused by the nuclear type of Citrus leprosis virus (CiLV-N). Virus Review and Research, Rio de Janeiro, suppl. 15: on line.
KITAJIMA, E.W., CHAGAS, C.M., BRAGHINI, M.T., FAZUOLI, L.C., LOCALI-FABRIS, E.C., SALAROLI, R.B. 2011b. Natural infection of several Coffea species and hybrids and Psilanthus ebracteolatus by the coffee ringspot virus (CoRSV). Scientia Agricola, Piracicaba, vol. 68, pp. 503-507.
KITAJIMA, E.W. & ALBERTI, G. 2014. Anatomy and fine structure of Brevipalpus mites (Tenuipalpidae) – Economically important plant-virus vectors. Part 7. Ultrastructural detection of cytoplasmic and nuclear types of Brevipalpus-transmitted viruses. Zoologica, Stuttgart, vol. 160, pp. 173-192.
KITAJIMA, E.W., NOVELLI, V.M. & ALBERTI, G. 2014. Anatomy and fine structure of Brevipalpus mites Tenuipalpidae) – Economically important plant-virus vectors. Part 1: An update on the biology and economical importance of Brevipalpus mites. Zoologica, Stuttgart, vol. 160, pp. 1-10.
KITAJIMA, E.W., TASSI, A.D., CACERES, S., AGUIRRE, A., COSTA, N., CALEGARIO, R.F. 2015. Host range, vector and geographic distribution of a Brevipalpus- transmitted virus causing ringsots in Ligustrum spp. Virus Review & Research, Rio de Janeiro, suppl .20, pp. 196-197.
LOCALI-FABRIS, E. C.; FREITAS-ASTÚA, J.; MACHADO, M. A. 2011. Genus Cilevirus. In Virus Taxonomy - Ninth Report of the International Committee on Taxonomy of Viruses; KING, A.; ADAMS, M.; CARSTENS, E.; LEFKOWITZ, E., Eds.; Elsevier/Avademic Press: London, United Kingdom, pp. 1139–1142.
MARQUES, J.P.R., FREITAS-ASTUA, J., KITAJIMA, E.W. & APEZZATTO DA GLÓRIA, B. 2007. Lesões foliares e de ramos de laranjeira-doce causadas pela leprose-dos-citros. Pesquisa Agropecuária Brasileira, BrasÃlia, vol. 42, n. 11, pp. 1531-1536.
MARQUES, J. P. R., KITAJIMA, E. W., FREITAS-ASTÚA, J. & APEZZATTO-DA-GLORIA, B. , 2010.Comparative morphoanatomical studies of the lesions caused by citrus leprosis virus on sweet orange. Anais da Academia Brasileira de Ciências, Rio de Janeiro, 82: 501-511
RAMOS-GONZÃLEZ. P.L. , CHABI-JESUS, C., GUERRA-PERAZA, O., TASSI, A.D., KITAJIMA, E.W., HARAKAVA, R., SALAROLI, R.B., FREITAS-ASTÚA, J. 2017. Citrus leprosis virus N: a new dichorhavirus causing citrus leprosis disease. Phytopathology, St. Paul, vol. 107, pp. 963-976.
RAMOS-GONZÃLEZ, P.L.; CHABI-JESUS, C.; BANGUELA-CASTILLO, A.;, TASSI, A.D.; RODRIGUES, M.C.; KITAJIMA, E.W.; HARAKAVA, R.; FREITAS-ASTÚA, J. Unveiling the complete genome sequence of clerodendrum chlorotic spot virus, a putative dichorhavirus infecting ornamental plants. Archives of Virology, Berlin, 163: 2519-2524. 2018.
ROY, A.; CHOUDHARY, N.; GUILLERMO, L. M.; SHAO, J.; GOVINDARAJULU, A.; ACHOR, D.; WEI, G.; PICTON, D. D.; LEVY, L.; NAKHLA, M. K.; HARTUNG, J. S.; BRLANSKY, R. H.A . 2013. Novel virus of the genus Cilevirus causing symptoms similar to citrus leprosis. Phytopathology, St. Paul, vol. 103, pp. 488–500.
ROY, A.; STONE, A. L.; SHAO, J.; OTERO-COLINA, G.; WEI, G.; CHOUDHARY, N.; ACHOR, D.; LEVY, L.; NAKHLA, M.K.; HARTUNG, J. S.; SCHNEIDER, W. L.; BRLANSKY, R. H. 2015. Identification and molecular characterization of nuclear citrus leprosis virus, a member of the proposed Dichorhavirus genus infecting multiple citrus species in Mexico. Phytopathology, St. Paul, vol. 105, pp. 564–75.
SAKAI, W.S. 1973. Simple method for differential staining of paraffin embedded plant 396 material using toluidine blue O. Stain Technology, Baltimore, vol. 48, n. 5, pp 247–249.
