TRANSMISSION OF Sclerotinia sclerotiorum FROM SOYBEAN SEED TO SEEDLINGS

Autores

  • Cristiane de Carvalho Universidade de Sorocaba - UNISO Agronomia
  • Victor Augusto Forti Universidade Metodista de Piracicaba
  • Mauro Ferreira Bonfim Junior Universidade de São Paulo/Escola Superior de Agricultura "Luiz de Queiroz"
  • Maria Heloisa Duarte Moraes Universidade de São Paulo/Escola Superior de Agricultura "Luiz de Queiroz"
  • José Otávio Machado Menten Universidade de São Paulo/Escola Superior de Agricultura "Luiz de Queiroz"

DOI:

https://doi.org/10.37856/bja.v91i1.238

Resumo

Sclerotinia sclerotiorum causes disease in a wide range of plants and their seeds are an important vehicle of disease dissemination. The transmission of this fungus from seeds to seedlings is still understudied, thus, this paper evaluated the transmission of S. sclerotium from infected soybean seeds to seedlings. Soybean seeds were cultured in contact with pathogen colonies for 18 and 24h; 50% of each sample was submitted to asepsis. To evaluate the transmission, the percentage and speed of emergence of the seedlings was recorded, then the emerged seedlings with/or without symptoms were harvested, cut into two segments and incubated on agar-bromophenol to confirm S. sclerotium transmission.  Results showed that a lower emergence percentage and speed was obtained after 24h inoculation without asepsis; the transmission of S. sclerotiorum therefore occurred from soybean seeds to symptomatic seedlings. Asymptomatic seedlings do not present the fungus, indicating there was no transmission.

Biografia do Autor

Cristiane de Carvalho, Universidade de Sorocaba - UNISO Agronomia

Etec "Martinho di Ciero"

Victor Augusto Forti, Universidade Metodista de Piracicaba

 Faculdade de Ciências Exatas e da Natureza

Mauro Ferreira Bonfim Junior, Universidade de São Paulo/Escola Superior de Agricultura "Luiz de Queiroz"

Dep.de Fitopatologia e Nematologia

Maria Heloisa Duarte Moraes, Universidade de São Paulo/Escola Superior de Agricultura "Luiz de Queiroz"

Dep.de Fitopatologia e Nematologia

José Otávio Machado Menten, Universidade de São Paulo/Escola Superior de Agricultura "Luiz de Queiroz"

Dep.de Fitopatologia e Nematologia

Referências

AGRIOS, G.N. 2005. Plant Pathology. San Diego: Elsevier Academic Press, 5th ed. 635p.

BOLTON, M.D., THOMMA, B.P.H.J.; NELSON, B.D. 2006. Sclerotinia sclerotiorum (Lib.) de Bary: biology and molecular traits of a cosmopolitan pathogen. Molecular Plant Pathology, London, v.7, n.1, p.1-16. http://onlinelibrary.wiley.com/doi/10.1111/j.1364-3703.2005.00316.x/full

BOTELHO, L.S., MORAES, M.H.D.; MENTEN, J.O.M. 2008. Fungi associated to the seeds of ipê-amarelo (Tabebuia serratifolia) and ipê-roxo (Tabebuia impetiginosa): incidence, germination effect and seedlings transmission. Summa Phytopathologica, Jaguariuna, v. 34, n.4, p. 343-348. http://www.scielo.br/pdf/sp/v34n4/v34n4a08.pdf

HENNEBERG, L.; JACCOUD FILHO, D. S.; RUARO, L.; PANOBIANCO, M. 2012. Efficiency of methods to detect Sclerotinia sclerotiorum in commercial soybean seed lots. Revista Brasileira de Sementes, Londrina, v.34, n.1, p.61-69. http://www.scielo.br/scielo.php?pid=S0101-31222012000100008&script=sci_arttext

HOFFMAN, D.D., HARTMAN, G.L., MUELLER, D.S., LEITZ, C.V., NICKELL, C.D.; PEDERSEN, W.L. 1998. Yield and seed quality of soybean cultivars infected with Sclerotinia sclerotiorum. Plant Disease, Saint Paul, v.82, n.7, p.826-828. http://www.ars.usda.gov/SP2UserFiles/ad_hoc/54000000WhiteMoldResearch/PublicationPDFs/YieldandSeedQualityofSoybeanCultivars.pdf

KIM, K.S., MIN, J.Y.; DICKMAN, M.B. 2008. Oxalic acid is an elicitor of plant programmed cell death during Sclerotinia sclerotiorum disease development. Molecular Plant-Microbe Interactions, Saint Paulv.21, n.5, p. 605–612. http://dx.doi.org/10.1094/MPMI-21-5-0605

KIMATI, H.; AMORIM, L.; REZENDE, J.A.M.; BERGAMIM FILHO, A.; CAMARGO, L.E.A. 2005. Manual de fitopatologia: doenças de plantas cultivadas. São Paulo: Agronômica Ceres, 4ªed. 663p.

KOCH, E.F.A. 2001. Effect of Sclerotinia sclerotiorum on the initial growth of common bean seedlings. Summa Phytopathologica, Jaguariuna, v. 27, p. 68-73.

LIVINGSTONE, D.M.; HAMPTON, J.L.; PHIPPS, P.M.; GRABAU, E.A. 2005. Enhancing resistance to Sclerotinia minor in peanut by expressing a barley oxalate oxidase gene. Plant Physiology, Washington, v.137, n.4, p.1354-1362. http://www.plantphysiol.org/content/137/4/1354.full

MAGUIRE, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science,Madison v.2, n.2 p.176-177.

SAHARAN, G.S.; MEHTA, N. 2008. Sclerotinia diseases of crop plants: biology, ecology and disease management. London: Springer, 486p. http://download.springer.com/static/pdf/583/bfm%253A978-1-4020-8408-9%252F1.pdf?auth66=1419853024_65f3e59d1c1b40379fd8fc89a2e9b7ca&ext=.pdf

STEADMAN, J.R., MARCINKOWSKA, J.; RUTLEDGE, S. 1994. A semi-selective medium for isolation of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology, Ontario, v.16, n.1, p.68-70.

TU, J.C. 1988. The role of white mold-infected white bean (Phaseolus vulgaris L.) seeds in the dissemination of Sclerotinia sclerotiorum (Lib.) de Bary. Journal of Phytopathology, Berlin, v.121, n.1, p.40-50.

Downloads

Publicado

2016-05-09

Edição

Seção

Artigos