𝘉𝘢𝘤𝘪𝘭𝘭𝘶𝘴 𝗌𝗉. RZ2MS9 AND THE BACTERIA-FREE FILTRATE IN THE SEED GERMINATION AND GROWTH OF MAIZE SEEDLINGS

Autores

  • Pedro Avelino Maia de Andrade Universidade Federal do Amazonas
  • Lucas Smith Pimenta Federal University of Amazonas (UFAM), Department of Biotechnology, post-code 69080-900 Manaus, AM, Brazil
  • Bruno Ewerton da Silveira Cardillo Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Plant Production, post-code 13418-900 Piracicaba, SP, Brazil
  • Joelma Marcon Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Genetics, post-code 13418-900 Piracicaba, SP, Brazil
  • José Antônio da Silva Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Genetics, post-code 13418-900 Piracicaba, SP, Brazil
  • João Lucio de Azevedo Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Genetics, post-code 13418-900 Piracicaba, SP, Brazil
  • Ana Dionisia da Luz Coelho Novembre Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Plant Production, post-code 13418-900 Piracicaba, SP, Brazil
  • Maria Carolina Quecine Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Genetics, post-code 13418-900 Piracicaba, SP, Brazil

DOI:

https://doi.org/10.37856/bja.v95i2.4232

Resumo

Seed vigor and seedling growth directly impact the early stages of maize production. These traits might be improved with the use of bioinoculants. This work aimed to evaluate the influence of Bacillus sp. RZ2MS9 and its bacteria-free filtrate in the seeds’ germination rate (G) and speed (GSI) and seedlings’ length (SL) and dry mass (SDM) of two maize hybrids. After receiving experimental treatments, seeds of maize hybrids DKB390 and 30A37PW® were grown at a germinator at 25oC and 90% humidity. GSI was evaluated daily while G (%), SL (cm) and SDM (mg.10 seeds-1) were evaluated after 7 days. GSI and SDM were higher in both hybrids treated with Bacillus sp. RZ2MS9. The bacteria-free filtrate produced higher GSI in the 30A37PW® hybrid only in comparison to the control treatments. Thus, the Bacillus sp. RZ2MS9 and its extracellular secreted compounds might comprise alternative tools to improve development and production of maize plants.

Biografia do Autor

Pedro Avelino Maia de Andrade, Universidade Federal do Amazonas

Contribuiu com: Concepção e desenho experimental, Coleta de amostras, Condução do experimento, Análise de dados, Escrita e Revisão do manuscrito.

Lucas Smith Pimenta, Federal University of Amazonas (UFAM), Department of Biotechnology, post-code 69080-900 Manaus, AM, Brazil

Contribuiu com: Coleta de amostras, Condução do experimento, Análise de dados, Escrita e Revisão do manuscrito.

Bruno Ewerton da Silveira Cardillo, Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Plant Production, post-code 13418-900 Piracicaba, SP, Brazil

Contribuiu com: Coleta de amostras, Condução do experimento, Análise de dados, Escrita e Revisão do manuscrito.

Joelma Marcon, Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Genetics, post-code 13418-900 Piracicaba, SP, Brazil

Contribuiu com: Coleta de amostras, Condução do experimento, Análise de dados, Escrita e Revisão do manuscrito.

José Antônio da Silva, Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Genetics, post-code 13418-900 Piracicaba, SP, Brazil

Contribuiu com: Coleta de amostras, Condução do experimento, Análise de dados, Escrita e Revisão do manuscrito.

João Lucio de Azevedo, Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Genetics, post-code 13418-900 Piracicaba, SP, Brazil

Contribuiu com: Concepção e desenho experimental, Análise de dados, Fornecimento de reagentes/materiais/ferramentas de análise, Escrita e Revisão do manuscrito.

Ana Dionisia da Luz Coelho Novembre, Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Plant Production, post-code 13418-900 Piracicaba, SP, Brazil

Contribuiu com: Coleta de amostras, Condução do experimento, Análise de dados, Fornecimento de reagentes/materiais/ferramentas de análise, Escrita e Revisão do manuscrito.

Maria Carolina Quecine, Luiz de Queiroz College of Agriculture - University of São Paulo (ESALQ-USP), Department of Genetics, post-code 13418-900 Piracicaba, SP, Brazil

Contribuiu com: Concepção e desenho experimental, Análise de dados, Fornecimento de reagentes/materiais/ferramentas de análise, Escrita e Revisão do manuscrito.

Referências

BARAZANI, O.; FRIEDMAN, E. 2000. Effect of exogenously applied L-tryptophan on allelochemical activity of plant growth-promoting rhizobacteria (PGPR ). Journal of Chemical Ecology, v.26, n.2, p.343-349. https://doi.org/10.1023/A:1005449119884.

BASHAN, Y.; DE-BASHAN, L. E.; PRABHU, S. R.; et al. 2014. Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013). Plant and Soil, v.378, p.1–33. https://doi.org/10.1007/s11104-013-1956-x.

BATISTA, B. D.; LACAVA, P. T.; FERRARI, A.; TEIXEIRA-SILVA, N. S.; BONATELLI, M. L.; TSUI, S.; MONDIN, M.; KITAJIMA, E.W.; PEREIRA, J.O.; AZEVEDO, J.L.; QUECINE , M.C. 2018. Screening of tropically derived, multi-trait plant growth-promoting rhizobacteria and evaluation of corn and soybean colonization ability. Microbiological Research, v.206, p.33–42. https://doi.org/10.1016/j.micres.2017.09.007.

CASSÃN, F.; PERRIG, D.; SGROY, V.; MASCIARELLI, O.; PENNA, C.; LUNA , V. 2009. Azospirillum brasilense Az39 and Bradyrhizobium japonicum E109, inoculated singly or in combination, promote seed germination and early seedling growth in corn (Zea mays L.) and soybean (Glycine max L.). European Journal of Soil Biology, v. 45, n.1, p.28–35. https://doi.org/10.1016/j.ejsobi.2008.08.005.

DAS, S.; DASH, D.; GUPTA, S. B.; DEOLE, S. 2018. Study of characterization of tamarind associated Rhizobium spp. and phosphate solubilizing bacteria and their potency for germination of tamarind seeds. The Pharma Innovation Journal, v.7, n.11, p.282–286. Available at: http://www.thepharmajournal.com/archives/2018/vol7issue11/PartE/7-10-110-931.pdf Access in: April 15 , 2020

FIGUEIRA, C; FERREIRA, M. J.; SILVA, H.; CUNHA, A. 2019. Improved germination efficiency of Salicornia ramosissima seeds inoculated with Bacillus aryabhattai SP1016-20. Annals of Applied Biology, v.174, n.3, p.319–328. https://doi.org/10.1111/aab.12495.

HU, Q.; LIU, R.; LIU, J. 2019. Effects of Bacillus subtilis QM3 on Germination and Antioxidant Enzymes Activities of Wheat Seeds under Salt Stress. Open Access Library Journal, v.6, n.3, p.1–9. https://doi.org/10.4236/oalib.1105218.

KARTHIKEYAN, B.; JALEEL, C. A.; GOPI, R.; DEIVEEKASUNDARAM, M. 2007. Alterations in seedling vigour and antioxidant enzyme activities in Catharanthus roseus under seed priming with native diazotrophs. Journal of Zhejiang University – SCIENCE B, v.8, n.7, p.453-457. https://doi.org/10.1631/jzus.2007.B0453.

LEE, H.S.; MADHAIYAN, M.; KIM, C.W.; CHOI, S.J.; CHUNG, K.Y. 2006. Physiological enhancement of early growth of rice seedlings (Oryza sativa L.) by production of phytohormone of N2-fixing methylotrophic isolates. Biology and Fertility of Soils, Berlin, v.42, p.402-408. https://doi.org/10.1007/s00374-006-0083-8.

LI, Z.; GUO, B.; WAN, K.; CONG, M.; HUANG, H.; GE, Y. 2015. Effects of bacteria-free filtrate from Bacillus megaterium strain L2 on the mycelium growth and spore germination of Alternaria alternata. Biotechnology and Biotechnological Equipment, v.29, n.6, p.1062–1068. https://doi.org/10.1080/13102818.2015.1068135.

MOTERLE, L. M.; SANTOS, R. F.; SCAPIM, C. A.; de LUCCA e BRACCINI, A.; BONATO, C. M.; CONRADO , T. 2011. Effect of plant growth regulator on germination and vigor of soybean seeds. Revista Ceres, v.58, n.5, p.651–660. https://doi.org/10.1590/S0034-737X2011000500017.

NAKAGAWA, J. Testes de vigor baseados no desempenho das plântulas. In: Krzyzanowski, F. C.; Vieira, R. D.; França Neto, J. B. (Ed.). Vigor de sementes: conceitos e testes. Londrina: ABRATES, 1999, cap.2.1, p.2-24.

NARDI, S.; PIZZEGHELLO, D.; SCHIAVON, M.; ERTANI, A. 2016. Plant biostimulants: Physiological responses induced by protein hydrolyzed-based products and humic substances in plant metabolism. Scientia Agricola, Piracicaba, Brazil, v.73, n.1, p.18–23. https://doi.org/10.1590/0103-9016-2015-0006.

NOUMAVO, P. A.; KOCHONI, E.; DIDAGBÉ, Y. O.; ADJANOHOUN, A.; ALLAGBÉ, M.; SIKIROU, R.; GACHOMO, E.W.; KOTCHONI, S.O.; BABA-MOUSSA , L. 2013. Effect of Different Plant Growth Promoting Rhizobacteria on Maize Seed Germination and Seedling Development. American Journal of Plant Sciences, v.4, n.5, p.1013–1021. http://dx.doi.org/10.4236/ajps.2013.45125.

RAMAMOORTHY, K.; NATARAJAN, N.; KILLIKULAM, T. N. A. U.; VALLANAD, P.O. 2000. Seed biofortification with Azospirillum spp. for improvement of seedling vigour and productivity in rice (Oryza sativa L.). Seed Science and Technology, Bassersdorf, Switzerland, v.28, n.3, p. 809-815. Available at: https://www.cabdirect.org/cabdirect/abstract/20013009232 Access in: March 13, 2020.

RAVEN, P. H.; EVERT, R. F.; EICHHORN, S.E. 2007. Biologia vegetal 7a ed. Rio de Janeiro, Guanabara Koogan, 856p.

SCHLINDWEIN, G.; VARGAS, L. K.; LISBOA, B. B.; AZAMBUJA, A.C.; GRANADA, C . E.; GABIATTI, N.C.; PRATES, F.; STUMPF, R. 2008. Influence of rhizobial inoculation on seedling vigor and germination of lettuce. Ciência Rural, v.38, n.3, p.658-664. https://doi.org/10.1590/S0103-84782008000300010.

SILVA, R. D. A.; SANTOS, J.L.; OLIVEIRA, L. S.; SOARES, M. R. S.; SANTOS, S. M. S. 2016. Biostimulants on mineral nutrition and fiber quality of coton crop. Revista Brasileira de Engenharia Agrícola e Ambiental, v.20, n.12, p.1062–1066. https://doi.org/10.1590/1807-1929/agriambi.v20n12p1062-1066.

TAVANTI, T.R.; TAVANTI, R.F.R.; GALINDO, F.S.; SIMÕES, I.; DAMETO, L.S.; de SÃ, M.E. 2020. Yield and quality of soybean seeds inoculated with Bacillus subtilis strains. Revista Brasileira de Engenharia Agrícola e Ambiental, v. 24, n.1, p. 65-71. https://doi.org/10.1590/1807-1929/agriambi.v24n1p65-71.

VINHAL-FREITAS, I. C.; SEGUNDO, J. P.; SILVA, M.; ITUIUTABA, E. 2011. Germinação e vigor de sementes de soja classificadas em diferentes tamanhos. Agropecuária Técnica, v.32, n.1, p.108–114. https://doi.org/10.25066/agrotec.v32i1.9567.

YAKHIN, O. I.; LUBYANOV, A. A.; YAKHIN, I. A.; BROWN, P. H. 2017. Biostimulants in plant science: A global perspective. Frontiers in Plant Science, v.7, 2049 p. https://doi.org/10.3389/fpls.2016.02049.

Downloads

Publicado

2020-08-26

Edição

v. 95 n. 2 (2020)

Seção

Artigos